The Argentina forward has again been ruled out with injury for Paris Saint-Germain as they host Montpellier in Ligue 1.
Lionel Messi also missed PSG’s win over Metz on Wednesday 22 September.
He will once more be absent just days before a potentially crucial Champions League clash with Manchester City.
Former Barcelona Star was taken off by Mauricio Pochettino during last weekend’s win over Lyon.
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“Lionel Messi started running again today in line with his treatment plan,” said PSG in announcing Messi‘s absence from the squad for the game against Montpellier.
A further check is scheduled for Sunday.
Marco Verratti will also miss the encounter and will begin training with the squad next week.
While Sergio Ramos is nearing a debut after his summer move from Real Madrid.
Messi is yet to score for his new club after leaving a financially-stricken Barcelona this summer.
Bringing to a close a more than 20-year association with the Catalan club.